Elementary Study on Anther Culture of Pansy

The effects of different ways of bud sterilization,bud cold pretreatment,different combinations of plant growth regulators on callus induction rate of anther and plant regeneration of Pansy were investigated.The results showed that 20 min with 0.1% HgCl2 was an ideal bud surface sterilization time,and obvious difference was detected between two genotypes in their effect on callus initiation of anther,but the overall rate of callus induction was not high.It may be suggested that the kind and concentration of hormones and culture conditions should be key factors for successful anther culture of pansy.